IL-6 antibody

Catalog No. ABIN2665190

This anti-IL-6 antibody is a Goat Polyclonal antibody detecting IL-6 in FACS. Suitable for Rat.

Quick Overview for IL-6 antibody (ABIN2665190)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: Poly5177

Product Details anti-IL-6 Antibody

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background: Interleukin 6 (IL-6) is a prototypic member of the IL-6 superfamily of cytokines. IL-6 is secreted by a variety of lymphoid and non-lymphoid cells including T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, keratinocytes, etc. Mature Rat IL-6 is a 187 aa residue protein. At the protein sequence level, there is approximately 39 % identity between rat and human IL-6, and 87 % identity between mouse and rat IL-6. The production of IL-6 can be induced by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines, and viruses. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a subunit (gp130) that acts as the universal signal-transducing element for all IL-6 family cytokines. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. One of the most important mechanism is that IL-6 is a key factor that reciprocally regulates Th17 and Foxp3+ Treg differentiation by inhibition of TGF-β induced Foxp3 and induction of RORγt, a Th17 lineage-specific transcription factor. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies.

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome